Physiological Intra-Cytoplasmic Sperm Injection(PICSI)
PICSI is a technique that simulates the natural selection of mature sperm. The principle of this method is the cultivation of mature sperm in a specially treated dish, to which a gel containing hyaluronan is applied. The sperm selected are then used for micro-manipulative fertilisation.
Mature, biochemically competent sperm bind to the hyaluronan where they can be isolated by the embryologist and used for ICSI. This procedure mimics a key step in the natural fertilization process, the binding of mature sperm to the oocyte complex. As a result, the selected sperm is essentially the same as one that would be successful in the natural reproductive process.The research proved that hyaluronan-bound PICSI-selected sperm are, in the vast majority of cases, more mature, exhibit less DNA damage, and have fewer chromosomal aneuploidyes.